Summer Islands Game Of Thrones, padding: 0 !important; border-bottom: 10px solid #33ac08; As a doctor please be confident and make sure that you have multiple pieces of evidence to back your diagnosis (claim). Typically an endocrinologist treats people with diabetes, metabolic disorders, growth disorders, thyroid disease and other related conditions. ELISA Procedure | Bio-Rad Studies have shown that these bodies are the most intense single marker for identifying persons with increased risk for diabetes development. Validity of screening for individuals at risk for type I diabetes by combined analysis of antibodies to recombinant proteins. During an autoimmune - mediated Diabetes mellitus type 1 leads to T-cell mediated destruction of beta cells of pancreatic islets, reduced production of insulin and glucose metabolism. ELISAs tend to be the most sensitive immunoassays due to the binding characteristics of the antibodies and the amplification or different read-out systems used. During the period of one year, in CCUS's Organizational unit, Institute for Clinical Immunology, 80 samples of patients with anti GAD and IA2 antibodies were analyzed. line-height: 21px; The orally disintegrating tablet form of this medication may contain over 3 milligrams of phenylalanine per tablet. When Elisa leaves, he. Although ELISA is a powerful and well-characterized application, attempting to develop and optimize a specific assay can be difficult. (1 point) O to provide employees with the resources they need O to conduct a needs analysis to Port Of Karfell, The signal gener- Is the first color change from white to blue sometimes flawed? Both assays showed good agreement with IEM, with a Kappa value of 0.86 for ELISA and 0.85 for RT-PCR. 0000006061 00000 n Hum Immunol. Advertisement Survey 1. Csar and Elisa have an explosive confrontation in the season finale, in which he swears he had nothing to do with Sara's fall and actually manages to look sincere. And as Lizzie indicated, a positive result on an ELISA does not mean anything if the Western Blot is negative. /* Why do we use a secondary antibody in ELISA? - Quora When blood sugar levels are high, which usually occurs in diabetes, there is too much sugar in the fluid for the body to reabsorb, so some sugar . color: #f5853b; margin: 0 .07em !important; Incubate samples overnight at 4C or follow the manufacturer guidelines. Contact us (800) 823-1969 or help@amplify.com to get a new code. If you are diabetic, check your blood sugar levels on a regular basis while you are taking this medicine. line-height: 32px; What Makes Blood Glucose Go Up or Down? - Diabetes Self-Management Elisa is feeling tired because she has anemia. Middle School Science Review | Amplify font-weight: bold; During 2014, the samples of 80 patients from Clinical Center University Sarajevo (CCUS) Pediatrics clinic's, Endocrinology department were analyzed on anti-GAD and IA2 antibodies. The situation is more urgent for people with type 1 diabetes. The liver is another part of your body that is confused by diabetes. Are you getting closer to figuring out why your patient, Elisa, could be feeling so tired? This can be due to low blood sugar, high blood sugar, low blood pressure, retinopathy, vestibular disorder, medication side effects, or dehydration. what does the human body need to function. yes . Untreated Diabetes: Short- and Long-Term Complications - Verywell Health loyola academy high school basketball; west collierville middle school registration The latter exhibited the higher analytical . /* Diabetes risk rises after COVID, massive study finds - Nature 0000009678 00000 n It helps to keep your choice of beverage, such as tea . Theenzyme-linked immunosorbent assay (ELISA)is an immunological assay commonly used to measureantibodies,antigens, proteinsandglycoproteinsin biological samples. D. Representing a taxpayer at a hearing. $9851b. Diabetes Antibody Standardization Program: First assay proficiency evaluation in Diabetes. Then you add the patient sample t. 3. Students figure out:Elisa's cells are getting enough oxygen and amino acids, but not enough glucose. Test and Verification. The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. This technique originated in the 1980's and is most often used today in pathogen detection, but has broad applications across many fields (summarized in Box 1). Signs and symptoms of pregnancy vary by stage (trimester). 0000008201 00000 n Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA) Optimize signal intensity to detect the maximum number of samples. CER, Microbiome, Metabolism | Science - Quizizz [Prevalence of immunologic markers (Anti-GAD and Anti-IA2) in first-degree relatives of patients with type I diabetes in the greater area of So Paulo]. Type 2 diabetes is far and away the most common type of diabetes.About 95 percent of people with the disease have Type 2 diabetes. {"@context":"https://schema.org","@graph":[{"@type":"Organization","@id":"https://www.fondation-fhb.org/#organization","name":"Fondation F\u00e9lix Houphou\u00ebt-Boigny pour la recherche de la paix","url":"https://www.fondation-fhb.org/","sameAs":["http://www.facebook.com/fondationfhb","http://fondationfhb","http://www.youtube.com/fondationfhb","https://twitter.com/fondationfhb"],"logo":{"@type":"ImageObject","@id":"https://www.fondation-fhb.org/#logo","inLanguage":"en-GB","url":"https://www.fondation-fhb.org/wp-content/uploads/2019/03/logo_ffhb.png","contentUrl":"https://www.fondation-fhb.org/wp-content/uploads/2019/03/logo_ffhb.png","width":108,"height":56,"caption":"Fondation F\u00e9lix Houphou\u00ebt-Boigny pour la recherche de la paix"},"image":{"@id":"https://www.fondation-fhb.org/#logo"}},{"@type":"WebSite","@id":"https://www.fondation-fhb.org/#website","url":"https://www.fondation-fhb.org/","name":"F\u00e9lix Houphou\u00ebt-Boigny Foundation for Peace Research","description":"","publisher":{"@id":"https://www.fondation-fhb.org/#organization"},"potentialAction":[{"@type":"SearchAction","target":"https://www.fondation-fhb.org/?s={search_term_string}","query-input":"required name=search_term_string"}],"inLanguage":"en-GB"},{"@type":"WebPage","@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#webpage","url":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/","name":"why does elisa have diabetes amplify - F\u00e9lix Houphou\u00ebt-Boigny Foundation for Peace Research","isPartOf":{"@id":"https://www.fondation-fhb.org/#website"},"datePublished":"2021-05-21T04:48:28+00:00","dateModified":"2021-05-21T04:48:28+00:00","breadcrumb":{"@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#breadcrumb"},"inLanguage":"en-GB","potentialAction":[{"@type":"ReadAction","target":["https://www.fondation-fhb.org/2021/05/21/ljvxentm/"]}]},{"@type":"BreadcrumbList","@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"item":{"@type":"WebPage","@id":"https://www.fondation-fhb.org/en/home/","url":"https://www.fondation-fhb.org/en/home/","name":"Home"}},{"@type":"ListItem","position":2,"item":{"@type":"WebPage","@id":"https://www.fondation-fhb.org/en/activities/","url":"https://www.fondation-fhb.org/en/activities/","name":"Activities"}},{"@type":"ListItem","position":3,"item":{"@type":"WebPage","@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/","url":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/","name":"why does elisa have diabetes amplify"}}]},{"@type":"Article","@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#article","isPartOf":{"@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#webpage"},"author":{"@id":""},"headline":"why does elisa have diabetes amplify","datePublished":"2021-05-21T04:48:28+00:00","dateModified":"2021-05-21T04:48:28+00:00","mainEntityOfPage":{"@id":"https://www.fondation-fhb.org/2021/05/21/ljvxentm/#webpage"},"commentCount":0,"publisher":{"@id":"https://www.fondation-fhb.org/#organization"},"articleSection":"Uncategorized","inLanguage":"en-GB","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https://www.fondation-fhb.org/2021/05/21/ljvxentm/#respond"]}]}]} trailer <]/Prev 461067/XRefStm 1554>> startxref 0 %%EOF 509 0 obj <>stream An ELISA (Enzyme-Linked ImmunoSorbant Assay) is a popular assay that uses antibodies and color change to detect proteins, peptides, antibodies or biomolecules in complex mixtures. font-size: 16px; Not taking enough of the diabetes medicines you've been prescribed. Concept: How does an ELISA allow the detection of | Chegg.com 2. color: #000000; HHS Vulnerability Disclosure, Help You can visit My Account to generate a new code. answer choices. 3Web The Elisa Amplification System Is Designed To Amplify The Amount Of Color Generated By A Given Quantity Of Immobilized Alkaline Phosphatase In A Soluble Substrate Enzyme. Youll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow. /* ]]> */ Huge number of patients with diabetes mellitus type 1 have these antibodies. Chernecky CC, et al. } background-color: #f5853b; 0000001918 00000 n Eye floaters and spots. [1] It is a form of high blood lipids and hyperlipoproteinemia (elevated levels of lipoproteins in the blood). margin-bottom: 0; In the long-term, diabetes increases the risk of damage to blood vessels and nerves . An ELISA test may be used to diagnose: HIV, which causes AIDS. One time you might actually have the option to do a direct or indirect assay is when you have all the reagents you need, but you need to amplify your signal sufficiently to detect low amounts. text-align: center; Diabetes risk rises after COVID, massive study finds Even mild SARS-CoV-2 infections can amplify a person's chance of developing diabetes, especially for those already susceptible to the. Part 1: Explaining a . An ELISA test has few risks because the test itself is performed on your blood sample in a lab. Figure 2. You can learn more about how we ensure our content is accurate and current by reading our. ELISAs are typically performed in 96-well or 384 . June 12, 2012 by Alex O'Meara. how to print avery 5395 labels in word; ELAST is based on the use of PerkinElmer's proprietary Tyramide Signal Amplification (TSA) technology. 0000004666 00000 n Haris B, Ahmed I, Syed N, Almabrazi H, Saraswathi S, Al-Khawaga S, Saeed A, Mundekkadan S, Mohammed I, Sharari S, Hawari I, Hamed N, Afyouni H, Abdel-Karim T, Mohammed S, Khalifa A, Al-Maadheed M, Zyoud M, Shamekh A, Elawwa A, Karim MY, Al-Khalaf F, Tatari-Calderone Z, Petrovski G, Hussain K. Sci Rep. 2021 Sep 23;11(1):18887. doi: 10.1038/s41598-021-98460-4. The enzyme linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. padding: 0 20px; Still stuck? 1973-2017 Fondation Flix Houphouet-Boigny pour la Recherche de la Paix. Published May 21, 2018. Indirect ELISA - Similar to direct ELISA, but the antibody is not conjugated. Without treatment, diabetes can lead to confusion, and possibly a loss of consciousness, coma, and death. First, you will explain how a healthy body functions. When Did The Humane Society Start, Welcome to the New NSCAA. You can also visit the library and use the Search tool at the top of the page. why does elisa have diabetes amplify how accurate is a negative elisa test 3 weeks post risky exposure for a woman (unprotected sex . .metaslider .caption { /* ]]> */ An enzyme-linked immunosorbent assay, also called ELISA or EIA, is a test that detects and measures antibodies in your blood.This test can be used to determine if you have . FOIA } Advertisement iajbwrjsoz Nice but have you heard of Higgs a whats that? which molecules do cells need to function. Summer Islands Game Of Thrones, Your email address will not be published. Take action. A common adaptation of indirect detection is to amplify the signal using avidin-biotin chemistry. Key Concept A functioning human body has molecules from food (glucose and amino acids) and molecules from air (oxygen) in its cells. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Although the test itself is relatively simple, waiting for the results or being screened for conditions such as HIV can cause a lot of anxiety. Sample volumes can also be adjusted when you have a very low abundant protein. I understand what molecules Elisa's cells need and where they come from. hbbRe`b``3 1 endstream endobj 476 0 obj <>/Metadata 45 0 R/PageLabels 42 0 R/Pages 44 0 R/StructTreeRoot 47 0 R/Type/Catalog/ViewerPreferences<>>> endobj 477 0 obj >/PageWidthList<0 612.0>>>>>>/Resources<>/ExtGState<>/Font<>/ProcSet[/PDF/Text]/XObject<>>>/Rotate 0/StructParents 0/TrimBox[0.0 0.0 612.0 792.0]/Type/Page>> endobj 478 0 obj <> endobj 479 0 obj <> endobj 480 0 obj <>stream Diabetic hyperphagia is not the same thing as binge eating. Originally described by Engvall and Perlmann (1971), the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. metabolism Flashcards | Quizlet HIV-1/2 ELISA assays have evolved over a number of generations to maximize sensitivity and specificity. 2003;52:112836. alternatives. Expert Answer. See this image and copyright information in PMC. Bookshelf 1997;20:96570. border-bottom: 1px solid #ededed; Eating well and being physically active most days of the week can help you. Some potential risks of getting. Which activity is the responsibility of every employer? Verification of diving systems; Pressure Testing; Subsea Testing; Test Facilities; Chemical analysis. background-color: #eee; Published by on June 29, 2022. Insulin-dependent diabetes mellitus during alpha-interferon therapy for chronic viral hepatitis. [CDATA[ */ Man City Vs Borussia Monchengladbach Prediction, Use of horseradish peroxidase and alkaline phosphatase in Her digestive system should break down starch molecules into glucose molecules, which are small enough to get into cells, and her circulatory system should deliver the glucose to cells. Report an issue. An ELISA test may be used to diagnose: HIV, which causes AIDS. Federal government websites often end in .gov or .mil. ELISA tests; anti GAD antibodies; anti IA2 antibodies. circulatory system. .epyt-gallery-thumb { Q. the body system that transports molecules to and from all cells of the body. Why does there need to be a step to convert substrate from blue to yellow by the addition of Stop Solution? The accumulation of an excessive amount of body fat can cause type 2 diabetes, and the risk of type 2 diabetes increases linearly with an increase in body mass index. why does elisa have diabetes amplify kelly somers biography labtestsonline.org/understanding/features/methods/start/2/, cdc.gov/lyme/diagnosistesting/LabTest/TwoStep/EIA/index.html, You Could Have 48 Hours to Avoid Lyme Disease After a Tick Bites You. background-color: #87cefa; However, when I add the standards (as well as the. why did jasmine richardson kill her family. Knowing how diabetes affects your body can help you look after your body and prevent diabetic complications from developing. lose weight or stay at a healthy weight. What are the advantages of ELISA? Results: If you or a loved one have been diagnosed with alcohol use disorder, your doctor may suggest these treatment options to help you get back on track. government site. This antibody is labelled with an enzyme, usually horse radish peroxidase or alkaline phosphatase. Syphilis diagnosis is made by either nontreponemal or treponemal approaches, though in developing countries the diagnosis relies mostly on nonspecific tests due to several reasons. H\@}&uoA8 1)iz`X>Taw>utxilY_g},Ogemi&mwqOg'^c7[]ijZ]e/&oKZ?Ct{IL76Tur^kWk j>X,uRNq\0__W+-yC~g~G3'X^~DzYs@VfEpz:=~A#4B 4B 4B 4B 4B M7Ao g`o: g3pl O=(++++++++{PKE}*{PooDE'N( The increased risk of needing an amputation when you have diabetes has a few causes. 0000411563 00000 n To do this, you have to know exactly how many carbohydrate grams are in your mealcue carb counting! People get diabetes because they deserve it . border-bottom: 10px solid #33ac08; Best Hotels In Boston With A View, Antibodies are proteins that your body produces in response to harmful substances called antigens. why Elisa does or does not have one of the four conditions. Verywell / Laura Porter. If your blood contains antibodies to the antigen, the two will bind together. Type 2: Because people with type 2 diabetes are resistant . Why do people get diabetes? reducing stress. B. cytokines. Alcohol can lower blood sugar level and put them at risk. Rather, having a drink alongside a meal is a much safer option. In people with type 2 diabetes, these complications can include heart disease, kidney damage, peripheral neuropathy (nerve pain), or vision loss. 0000004040 00000 n This site needs JavaScript to work properly. With diabetes, the body either does not make enough insulin or cannot use the insulin that it has effectively. Helps with weight loss. why does elisa have diabetes amplify. why does elisa have diabetes amplify - bestgranitestone.com Vocabulary Digital Tools Metabolism Modeling Tool activities: 1.3 Warm-Up and 1.3 Molecules in a Cell Metabolism Sorting Tool activity: 1.3 Evaluating Evidence Causes of fatigue can include high or low blood sugar levels, depression, being overweight, certain medications, and coinciding medical . The negative WB means that you do not have antibodies against HIV proteins in your blood, proving that you are not infected. exercising for at least 30 minutes daily. 2. In other words, the mender's attempt to communicate with Elisa goes down the drain mainly because she is so absorbed in her chrysanthemums. HIV-1/2 ELISA assays have evolved over a number of generations to maximize sensitivity and specificity. If you are a current Amplify customer, you can log in to your program from the list below. Here is the classic illustration of how this type of ELISA works. margin: 1em 0; Why Elisa Has Diabetes ( Or diabetes mellitus) By Elmedin Karamovic and Johnny Bild Why does Elisa Feel Tired all the Time Why Does Elisa Feel Tired All The Time? In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. Take action. 0000412527 00000 n Some of the advantages of using indirect Elisa: Offers high sensitivity and flexibility as the number of secondary antibodies can bind to a primary antibody and one type of secondary antibody can label different primary antibodies It is cheaper as there is a requirement of fewer labeled antibodies. When the kidneys no longer process the blood sugar (glucose) it essentially flushes it through the urine. By merging an ELISA with the Polymerase Chain Reaction (PCR), immuno-PCR provides extremely high levels of assay sensitivity. Out of total number of samples, 41 were male patients, or 51% and 39 female, or 49%. why does elisa have diabetes amplify - mkstampconcrete.com 0000000996 00000 n ?#^b7[]O)[5|.})!b~L7:Tur^kW:]s. Keywords: 2 1 understand how those molecules get to the cells in Elisa's body. } (800) 823-1969 or help@amplify.com. line-height: 29px; digestive system. border-collapse: collapse; Diabetic ketoacidosis - Symptoms and causes - Mayo Clinic 0000012021 00000 n var _EPYT_ = {"ajaxurl":"https:\/\/www.fondation-fhb.org\/wp-admin\/admin-ajax.php","security":"58ef36594c","gallery_scrolloffset":"20","eppathtoscripts":"https:\/\/www.fondation-fhb.org\/wp-content\/plugins\/youtube-embed-plus\/scripts\/","eppath":"https:\/\/www.fondation-fhb.org\/wp-content\/plugins\/youtube-embed-plus\/","epresponsiveselector":"[\"iframe.__youtube_prefs_widget__\"]","epdovol":"1","version":"13.4.2","evselector":"iframe.__youtube_prefs__[src], iframe[src*=\"youtube.com\/embed\/\"], iframe[src*=\"youtube-nocookie.com\/embed\/\"]","ajax_compat":"","ytapi_load":"light","pause_others":"","stopMobileBuffer":"1","vi_active":"","vi_js_posttypes":[]}; Last medically reviewed on June 22, 2017, A Lyme disease antibody test is used to determine if you have contracted the bacterium that causes Lyme disease. HIV-1 ELISA assays were the earliest approved serologic tests for HIV infection and remain the most sensitive commercial assays for infection. font-size: 16px; But since Elisa's body is not working properly because she has diabetes there is not enough insulin in her body to let glucose into her cells which can leads to her only having a total of 19 molecules of glucose absorbed in her cells as stated in her test results. Detection antibody binds to any target antigen already bound to the plate. Size interference: In general alkaline phosphatase and HRP are large enzymes and thus size may interfere with the biochemical function of the protein to which they are attached. Signs of diabetes When undiagnosed or uncontrolled, the effects of [] To work out the concentration of antigen in a sample, a standard curve using a solution of known concentration needs to be prepared. A false-positive result indicates you have a condition when you actually dont. BMG LABTECH. .archive #page-title { } How much change the enzyme causes allows the technician to determine the presence and amount of antibody. Since the assay uses surface binding for separation, several washes are repeated between each ELISA step to remove unbound materials. , e to the IRS about a taxpayer #footer-widgets .widget a, #footer-widgets .widget a:visited { We avoid using tertiary references. c. Capture any evidence from this blood test result that you can use to help you make your final medical diagnosis. hb```b`` ``e``Wcf@ a, :8~14`)cu``V[t"i /cTx@!g[JcSw|C6"+fFXw|VHE/XIJ!_BsXg9 jW>IVd,3F>Fn@>J,;;+=8P n 1(WP(Gh`(3l%uds q&8}@l(p0r7N``p`$>q5n $M >& \1,s` a~fL0|c2`i+01_`Z`hL-%lw40U1 endstream endobj 508 0 obj <>/Filter/FlateDecode/Index[47 428]/Length 37/Size 475/Type/XRef/W[1 1 1]>>stream "People who have really poorly controlled diabetes are more susceptible to . 475 0 obj <> endobj xref Diabesity: How Obesity Is Related to Diabetes - Cleveland Clinic UNESCO Chair 8600 Rockville Pike why does elisa have diabetes amplify - Flix Houphout-Boigny margin: 0; .so-mobilenav-mobile + * { display: none; } 1. } Cesarini PR, Mendona E, Fernandes V, Silva Rdo C, Morimitsu LK, Garcia FE, Vechiatti S, Miranda WL, Dib SA. Contact us (800) 823-1969 or help@amplify.com. Would you like email updates of new search results? ELISA is often used as a screening tool before more in-depth tests are ordered. display: block; Fabris P, Betterle C, Greggio NA, Zanchetta R, Bosi E, Biasin MR, de Lalla F. J Hepatol. body.layout-full { Diabetic Hyperphagia: Causes, Symptoms, Treatments - Verywell Health 2017 Sep 19;114(38):10196-10201. doi: 10.1073/pnas.1711169114. First ( [1] ), the capture antibody is coated onto the plate and . They get diabetes because they don't exercise. margin-top: 40px; sharing sensitive information, make sure youre on a federal #colophon #theme-attribution, #colophon #site-info { blood, serum, biopsies). 1. font-weight: bolder; . The accumulation of an excessive amount of body fat can cause type 2 diabetes, and the risk of type 2 diabetes increases linearly with an increase in body mass index. font-size: 32px; PDF ELAST ELISA Amplification System - PerkinElmer Laboratory methods: enzyme-linked immunosorbent assay (ELISA). Comparison of ELISA and RT-PCR versus Immune Electron Microscopy for In Type 1 Diabetes mellitus is a result of T cell-mediated autoimmune disease during which the destruction of pancreatic isles beta cells occurs, followed by the lowered insulin production and glucose metabolism disorders, in genetically predisposed individuals ( 14 ). } Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum. why does elisa have diabetes amplify - enchelab.com line-height: 29px; how accurate is a negative elisa test 3 weeks post risky exposure for a woman (unprotected sex . -, Bingley PJ, Bonifacio E, Mueller PW. nervous system. That means poor blood sugar control . 0000005472 00000 n Low prevalence of GAD and IA2 antibodies in schoolchildren from a village in the southwestern section of the Netherlands. } When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. 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-moz-box-shadow: none; box-shadow: none } #footer-widgets .widget .widget-title { color: #ffffff } #footer-widgets .widget { color: #ffffff } #colophon .widget_nav_menu .menu-item a { border-color: #ffffff } #footer-widgets .widget a, #footer-widgets .widget a:visited { color: #ffffff } #footer-widgets .widget a:hover, #footer-widgets .widget a:focus, #footer-widgets .widget a:active { color: #ffffff } #colophon #theme-attribution, #colophon #site-info { color: #ffffff } #colophon #theme-attribution a, #colophon #site-info a { color: #ffffff }
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